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What is static light scattering (SLS)?


Static light scattering (SLS) is a technique that measures the average scattering intensities to calculate the weight-average molecular weight of particles in a liquid solution. When a laser beam irradiates macromolecules in solution, the macromolecules scatter light in all directions.


What is static light scattering SLS


The relationship between the scattering intensity and the molecular weight of a macromolecule is described by the simplified Rayleigh equation:


the simplified Rayleigh equation


The instrument collects scattering intensities of macromolecules in solutions at different sample concentrations. The Debye plot is then constructed by plotting and linearly fitting the Kc/R values versus the concentrations. The slope of the linear fit is used to calculate the second virial coefficient A2. And the Y-axis intercept of the linear fitting yields the reciprocal of molecular weight.


What static light scattering SLS is





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