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What are the factors that affect background?


The normal background signal is characterized by the following conditions:

  • The normal background should occupy less than 20 units of channel.
  • The higher signals should appear on the far left of the signal diagram.
  • The intensity of the background signal should be quite low.
  • The signal intensity decreases from left to right sequentially.
  • There should be no castellation in the signals as this could indicate bad alignment.
  • abnormal background signals vs normal background signal

The reasons for abnormal background signals vary. For example, the optical system alignment cannot be carried out; there may be impurities attached to the inner surface of the sample cell or the surface of the lens; the sample cell is foggy due to the temperature difference between the medium and the working environment, causing condensation on the sample cell; the medium is not pure; the working status of the laser source is not ideal; the lens or the sample cell are scratched. Troubleshooting should begin with inspecting the sample cell, then the laser source and lens, and finally, the alignment system.




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