BeNano 180 Zeta Pro

Unlock Greater Research Potential With BeNano 

 

• Advanced ELS Technology: PALS

PALS technology can effectively distingguish and extract the electrophoretic behavior even for sample with weak eletrophoretic mobilities, either close to isoelectrical point or with high salinity environment.

• Advanced DLS Technology: Backscattering Detection

Backscattering DLS optics can detect much larger scattering volume compared to 90-degree optics. Combined with movable measurement position, backscattering DLS offers much higher sensitivity and high turbidity sample measurement capacity. 

• Temperature Trend Measurement

For thermal sensitive samples, a temperature trend can be performed easily with a programmed SOP. The BeNano can detect the temperature transition point of the size results, which is the aggregation temperature for protein samples.

• Stable and Durable Optical Bench 

The BeNano adopts a 50mW solid-state laser, a singlemode fiber system and a high-performance APD detector, providing stable, wide-ranging, and highly redundant detection capabilities. 

• Research Level Software

The BeNano software can evaluate and process scattered light signals intelligently to improve the signal quality and result stability. Various built-in calculation modes can cover multiple scientific research and application fields.

• Ultra Low Sample Volume Required 

Measuring trace amount of sample is required for earlystage R&D in pharmaceutical industy and academia. With the capillary sizing cell, only 3 to 5 μL of sample is needed for precise size measurement.

 

1. Particle Size Measured by Dynamic Light Scattering (DLS) 

Dynamic light scattering (DLS), also referred to as photon correlation spectroscopy (PCS) or quasi-elastic light scattering (QELS), is a technique used to measure Brownian motion in a dispersant. It is based on the principle that smaller particles move faster while larger particles move slower. The scattering intensities of the particles are detected by an avalanche photodiode (APD) and then converted into a correlation function using a correlator. From this correlation function, a mathematic algorithm can be applied to obtain the diffusion coefficient (D). The hydrodynamic diameter (D_H) and its distribution can be calculated using the Stokes-Einstein equation, which relates the diffusion coefficient to the particle size.

 

2. Backscattering Detection Technology 

 

Features

• Wider Concentration Range

By optimizing the detection position, the highly concentrated samples can be detected near the edge of the sample cell, effectively minimizing the multiple light scattering effect.

• Higher Sensitivity

8-10 times scattering volume and around 10 times sensitivity as compared to the traditional 90° optical design.

• Higher Size Upper Limit

It mitigates multiple light scattering from large particles and, to some extent, reduces the number fluctuation of large particles due to the much larger scattering volume.

• Better Reproducibility

The DLS backscattering technology is less influenced by dust contaminants and unevenly distributed agglomerates and provides better reproducibility.

 

Intelligent Search for the Optimal Detection Position

• The software automatically determines the optimal detection position based on the size, concentration, and scattering ability of the sample to achieve the highest measurement accuracy and offer flexibility in detecting different types and concentrations of samples. This feature is particularly useful when dealing with a variety of samples, each with its unique scattering properties and concentrations.

 

(1) The detection point in the middle of the sample cell

This leads to a large scattering volume that increases instrument sensitivity and is suitable for detecting dilute samples with weaker scattering effects.

 

(2) The detection point at the edge of the sample cell

This avoids the multiple scattering effect of high concentration samples, ensuring accurate and repeatable particle size results.

 

3. DLS Flow Mode 

DLS flow mode provides a high-resolution size result of a complex, polydisperse system. When combined with front-end separation equipment such as GPC/SEC or FFF, particles are separated into monodisperse fractions and flow through the BeNano in sequence by size. The size of each fraction is continuously measured and summed into a high-resolution size distribution. 

 

BeNano can acquire RI or UV signals, offering a more accurate volume and number distributions independent of algorithm compared to a batch-mode measurement.

 

Integrated use of BeNano and SEC for particle characterization

 

Applications

• Characterizing particulates and polymers on size and dispersity
• Distinguishing monomers, dimers and aggregates of proteins

Effluent curves of size, intensity, and refractive index (RI)

Black Curve : BSA size distribution with batch mode 
Red Histograms : BSA size distribution with flow mode 
Flow mode provides more precise information for characterizing BSA molecules on size and dispersity compared with batch mode.

 

4. High-resolution Size Measurement 

 

Features

• DLS analyzer connecting with GPC/SEC, FFF, etc.
• Receiving up to 3 signals from RI, UV or other detectors 
• 27uL low-volume flow cell to avoid band broadening
• Size resolution as high as 1.3 : 1
• Size distributions weighted by number and volume besides intensity
• Suitable for complex, polydisperse systems such as proteins, polymers, etc.

High-resolution size distribution achieved through flow mode

Particles remain in flow during measurement

Following size separation, particles are detected

 

5. Zeta Potential Measured by Electrophoretic Light Scattering (ELS) 

In aqueous systems, charged particles are surrounded by counterions that form an inner Stern layer and an outer shear layer. Zeta potential is the electrical potential at the interface of the shear layer. A higher zeta potential indicates greater stability and less aggregation of the suspension system. Electrophoretic light scattering (ELS) measures electrophoretic mobility via Doppler shifts of scattered light, which can be used to determine the zeta potential of a sample by Henry's equation.

6. Phase Analysis Light Scattering (PALS) 

Phase analysis light scattering (PALS) is an advanced technology based on the traditional ELS technology, which has been further developed by Bettersize to measure the zeta potential and its distribution of a sample.

 

Features and Benefits 

• Accurate measurement of samples with low electrophoretic mobility
• Effective for samples in organic solvents with low dielectric constant
• More accurate results for samples with high conductivity  
• Effectively measures the zeta potential of particles whose charge approaches the isoelectric point

 

 

7. Static Light Scattering 

Static light scattering (SLS) is a technology that measures the scattering intensities, weight-average molecular weight (Mw), and second virial coefficient (A₂) of the sample through the Rayleigh equation: 

where c is the sample concentration, θ is the detection angle, R_θ is the Rayleigh ratio used to characterize the intensity ratio between the scattered light and the incident light at angle θ, Mw is the sample’s weight-average molecular weight, A₂ is the second virial coefficient, and K is a constant related to (dn/dc)².

During molecular weight measurements, scattering intensities of the sample at different concentrations are detected. By using the scattering intensity and Rayleigh ratio of a known standard (such as toluene), the Rayleigh ratios of samples at different concentrations are computed and plotted into a Debye plot. The molecular weight and the second virial coefficient are then obtained through the intercept and slope from the linear regression of the Debye plot.

 

 

8. Microrheology Measured by DLS 

Dynamic Light Scattering Microrheology (DLS Microrheology) is an economical and efficient technique that utilizes dynamic light scattering to determine rheological properties. By analyzing the Brownian motion of colloidal tracer particles, information about the viscoelastic properties of the system, such as viscoelastic modulus, complex viscosity and creep compliance, can be obtained with the generalized Stokes-Einstein equation.

Features & Benefits

• Investigates rheological behaviors by measuring the thermally-driven motion of tracer particles within a material being studied
• Facilitates the measurement across a wide range of frequencies
• Applies low stress to tracer particles
• Requires only a microliter-scale sample volume
• Complements mechanical rheology results
• Suitable for weakly-structured samples

 

9. Temperature Trend Measurement  

 

Measurement Parameters

• Size vs. Temperature 
• Zeta Potential vs. Temperature

 

Features

• Benefit protein formulation stability study
• Accelerates real-time aging through elevated temperature simulation

 

Benefits

• Easy examination of protein formulation stability 
• Accelerates real-time aging through elevated temperature simulation 

 

10. pH Trend Measurement  

 

Measurement Parameters

• Zeta Potential vs. pH
• Isoelectric point 
• Conductivity vs. pH 

 

Features

• High-precision ternary titration pumps
• Controllable peristaltic pump with high flow capacity and high flow rate
• General-purpose electrode
• Automated titrant selection based on initial and target pH using intelligent software

 

Benefits

• Completes measurements within a shorter time
• Improves consistency and repeatability of results
• Reduces the workload of researchers
• Simplifies qualifications needed for operators
• Accelerates real-time aging through elevated temperature simulation
• Reduces exposure to corrosive liquids

 

1. A Research Level Software 

• SOP guarantees measurement accuracy and completeness
• Automatic calculation of mean and standard deviation for results and statistics
• Comparison of results from multiple runs through statistics and overlay functions
• Real-time display of information and results
• Over 100 available parameters that meet research, QA, QC, and production needs
• Free lifelong upgrades provided

 

 

2. Compliance With FDA 21 CFR Part 11 

The BeNano software system is compliant with 21 CFR Part 11 regulations, which restricts access to authorized individuals through a username and password system for electronic record signing, access logs, change logs, or operation execution. An activation code can be used to upgrade security settings and ensure compliance, and an "audit trail" can be viewed to ensure proper management and maintenance of system security and data integrity.

 

 

 

3. Report Designer 

The built-in report designer provides a range of editable templates, enabling users to customize reports to their needs.

• 30 report templates cover all measurement results
• Flexible editing, one-click saving, and easy creation of userdefined reports
• Customizable report logo
  
  
  

 

Dynamic Light Scattering (DLS) -Applications	Electrophoretic Light Scattering(ELS) -Applications	Static Light Scattering(SLS) -Applications	DLS Microrheology -Applications
1) Particle size and distribution of polymers, colloids, biomacromolecules, etc. 2) Research on thermal-sensitive systems, such as PNIPAm polymer. 3) Studies on polymerization process and reaction mechanisms.  4) Kinetics  analysis of self-assembly, polymerization and depolymerization, etc.	1) Particle suspensions, includingcolloids, proteins, nanometals, etc. 2) Industries such as chemicals, biology, water treatment, paints,etc. 3) Product stability control and monitoring 4) Stability research and control of suspension system 5) Surface property and modification study	1) Chemical engineering:  characterization of polymers, micelles and macromolecules 2) Life science: characterization of proteins, polypeptides, and polysaccharides. 3) Pharmaceuticals:  research on aggregation and stability of drugs and biomacromolecules.	1) Polymer solution 2) Protein solution 3) Gel system

Functions	Parameter	BeNano 180 Zeta Pro	BeNano 180 Zeta	BeNano 90 Zeta	BeNano Zeta	BeNano 180 Pro	BeNano 180	BeNano 90
Size measurement	Size measurement range	0.3 nm - 15 μm*	0.3 nm - 10 μm*	0.3 nm - 15 μm*	N/A	0.3 nm - 15 μm*	0.3 nm -10 μm*	0.3 nm - 15 μm*
	Sample volume	3 μL - 1 mL*	40 μL - 1 mL*	3 μL - 1 mL*	N/A	3 μL - 1 mL*	40 μL - 1 mL*	3 μL - 1 mL*
	Detection angle	90° & 173° & 12°	173° & 12°	90° & 12°	N/A	90° & 173°	173°	90°
	Analysis algorithm	Cumulants, General Mode, CONTIN,  NNLS	Cumulants, General Mode, CONTIN,  NNLS	Cumulants, General Mode, CONTIN,  NNLS	N/A	Cumulants, General Mode, CONTIN,  NNLS	Cumulants, General Mode, CONTIN,  NNLS	Cumulants, General Mode, CONTIN,  NNLS
	Upper limit of concentration range	40% w/v*	40% w/v*	Optically clear+	N/A	40% w/v*	40% w/v*	Optically clear+
	Detection position	Movable position 0.4 - 5 mm	Movable position 0.4 - 5 mm	Fixed position 5 mm	N/A	Movable position 0.4 - 5 mm	Movable position 0.4 - 5 mm	Fixed position 5 mm
Zeta potential measurement	Detection angle	12°	12°	12°	12°	N/A	N/A	N/A
	Zeta potential measurement range	No actual limitation	No actual limitation	No actual limitation	No actual limitation	N/A	N/A	N/A
	Electrophoretic mobility	> ± 20 μm·cm/V·s	> ± 20 μm·cm/V·s	> ± 20 μm·cm/V·s	> ± 20 μm·cm/V·s	N/A	N/A	N/A
	Conductivity	0 - 260 mS/cm	0 - 260 mS/cm	0 - 260 mS/cm	0 - 260 mS/cm	N/A	N/A	N/A
	Sample volume	0.75 - 1 mL	0.75 - 1 mL	0.75 - 1 mL	0.75 - 1 mL	N/A	N/A	N/A
	Sample size	2 nm - 110 μm	2 nm - 110 μm	2 nm - 110 μm	2 nm - 110 μm	N/A	N/A	N/A
Other measurements	Molecular weight (Mw)	342 Da - 2 x 107 Da*	342 Da - 2 x 107 Da*	342 Da - 2 x 107 Da*	N/A	342 Da - 2 x 107 Da*	342 Da - 2 x 107 Da*	342 Da - 2 x 107 Da*
	Viscosity	0.01 cp - 100 cp*	0.01 cp - 100 cp*	0.01 cp - 100 cp*	N/A	0.01 cp - 100 cp*	0.01 cp - 100 cp*	0.01 cp - 100 cp*
	Interaction parameter KD	No actual limitation	No actual limitation	No actual limitation	N/A	No actual limitation	No actual limitation	No actual limitation
	Trend measurement	Time and temperature	Time and temperature	Time and temperature	Time and temperature	Time and temperature	Time and temperature	Time and temperature
System parameters	Temperature control range	-15℃ - 110℃, ±0.1℃	-15℃ - 110℃, ±0.1℃	-15℃ - 110℃, ±0.1℃	-15℃ - 110℃, ±0.1℃	-15℃ - 110℃, ±0.1℃	-15℃ - 110℃, ±0.1℃	-15℃ - 110℃, ±0.1℃
	Condensation control	Dry air or nitrogen	Dry air or nitrogen	Dry air or nitrogen	Dry air or nitrogen	Dry air or nitrogen	Dry air or nitrogen	Dry air or nitrogen
	Laser source	50 mW Solid-state laser, 671 nm#, Class 1	50 mW Solid-state laser, 671 nm#, Class 1	50 mW Solid-state laser, 671 nm#, Class 1	50 mW Solid-state laser, 671 nm#, Class 1	50 mW Solid-state laser, 671 nm#, Class 1	50 mW Solid-state laser, 671 nm#, Class 1	50 mW Solid-state laser, 671 nm#, Class 1
	Correlator	Up to 4000 channels, 1011 linear dynamic range	Up to 4000 channels, 1011 linear dynamic range	Up to 4000 channels, 1011 linear dynamic range	Up to 4000 channels, 1011 linear dynamic range	Up to 4000 channels, 1011 linear dynamic range	Up to 4000 channels, 1011 linear dynamic range	Up to 4000 channels, 1011 linear dynamic range
	Detector	Avalanche photodiode (APD)	Avalanche photodiode (APD)	Avalanche photodiode (APD)	Avalanche photodiode (APD)	Avalanche photodiode (APD)	Avalanche photodiode (APD)	Avalanche photodiode (APD)
	Intensity control	0.0001% - 100%, manual or automatic	0.0001% - 100%, manual or automatic	0.0001% - 100%, manual or automatic	0.0001% - 100%, manual or automatic	0.0001% - 100%, manual or automatic	0.0001% - 100%, manual or automatic	0.0001% - 100%, manual or automatic
	Dimensions (L x W x H)	62.5 x 40 x 24.5 cm (22 kg)	62.5 x 40 x 24.5 cm (22 kg)	62.5 x 40 x 24.5 cm (22 kg)	62.5 x 40 x 24.5 cm (22 kg)	62.5 x 40 x 24.5 cm (22 kg)	62.5 x 40 x 24.5 cm (22 kg)	62.5 x 40 x 24.5 cm (22 kg)
	Power supply	AC 100-240 V, 50-60 Hz, 4A	AC 100-240 V, 50-60 Hz, 4A	AC 100-240 V, 50-60 Hz, 4A	AC 100-240 V, 50-60 Hz, 4A	AC 100-240 V, 50-60 Hz, 4A	AC 100-240 V, 50-60 Hz, 4A	AC 100-240 V, 50-60 Hz, 4A
	Conformity to standards	21 CFR Part 11, ISO 13321, ISO 22412, ISO 13099	21 CFR Part 11, ISO 13321, ISO 22412, ISO 13099	21 CFR Part 11, ISO 13321, ISO 22412, ISO 13099	21 CFR Part 11, ISO 13099	21 CFR Part 11, ISO 13321, ISO 22412	21 CFR Part 11, ISO 13321, ISO 22412	21 CFR Part 11, ISO 13321, ISO 22412
* Dependent on samples and accessories + Up to 40% w/v using capillary sizing cell # 10mW 633nm He-Ne laser available on request

 

1. CUVETTES 

 

Standard
	Folded Capillary Cell BT-C1 For aqueous samples in zeta potential measurement Material: PC, Gold-plated Phosphor Bronze Sample Volume: 0.75 mL Temperature Range: -15 - 70 °C		PS Cuvette For aqueous samples in size measurement Material: Quartz Sample Volume: 1 - 1.5 mL Temperature Range: -15 - 70 °C
	Glass Cuvette  For aqueous and organic samples with better sealing performance in size measurement Material: Quartz Sample Volume: 1-1.5 mL Temperature Range: -15 - 110 °C		Capillary Sizing Cell For aqueous and organic samples with ultra-micro volume required in size measurement Material: Quartz Sample Volume: 3 - 5 μL Temperature Range: -15 - 70 °C
Optional
	Folded Capillary Cell BT-C1-Pt For aqueous samples in zeta potential measurement Material: PC, Platinum Sample Volume: 0.75 mL Temperature Range: -15 - 70 °C		Micro-volume Cuvette For aqueous samples with micro volume required in size measurement Material: PMMA Sample Volume: 40 - 50 μL Temperature Range: -15 - 70 °C
	Dip Cell For aqueous and organic samples in zeta potential measurement Material: PEEK, Platinum Sample Volume: 1 - 1.5 mL Temperature Range: -15 - 70 °C		Micro-volume Glass Cuvette For aqueous samples with micro volume required in size measurement Material: Quartz Sample Volume: 25 μL Temperature Range: -15 - 110 °C
	Flow Cell For aqueous and organic samples in DLS flow mode measurement Material: Quartz Sample Volume: 27 uL Temperature Range: 0 - 70 °C		Micro-volume Fluorescence-filtered Cell For aqueous samples in size measurement Material: Quartz Sample Volume: 16 uL  Temperature Range: 0 - 90 °C Measurement Angle: 90°, 173°
	Micro-volume VH Cell For aqueous samples in size measurement Eliminating the vertical element of scattered light Material: Quartz  Volume: 16 uL Temperature Range: 0 - 90 °C Measurement Angle: 90°		Micro-volume VV Cell For aqueous samples in size measurement Eliminating the horizontal element of scattered light Material: Quartz Sample Volume: 16 uL Temperature Range: 0 - 90 °C Measurement Angle: 90°, 173°

 

 

2. BAT-1 Autotitrator 

1) Introduction  

The BAT-1 autotitrator, a highly recommended accessory for the BeNano series, is an ideal option for analyzing the zeta potential as a function of pH and determining the isoelectric point (IEP) of a colloidal system. It is equipped with three high-precision titration pumps with a precision of 0.28 μL, and the system can select the optimal titrant and adjust the addition volume intelligently to optimize titration efficiency and accuracy.

2) Features 

• • Combination electrode with high precision and high feedback speed
  • High precision ternary titration pumps
  • Controllable peristaltic pump with high flow capacity and high flow rate
  • Internal magnetic stirrer system
  • SOP operation
  • Replaceable tubes
  • Corrosion resistant design
  • General purpose electrode
  • Intelligentization
  • Determination of isoelectric point
    
    

3) How it works 

The BAT-1 Autotitrator is designed to be used with the BeNano series for the measurement of zeta potential over a wide pH range, providing the information of zeta potentials and the stability of samples in different conditions. The operation flow is as follows:

 

a) Preparing the samples to be detected and the titrants in the containers, respectively;

 

b) Creating or editing a titration SOP in BeNano software by setting the volume of the sample to be measured, the concentrations of the titrants, the initial pH, the target pH, the pH interval and the target pH tolerance, etc..

 

c) To start the determination, the sample is titrated to approach the first pH value through automatic calculation, and is injected into the folded capillary cell by the peristaltic pump for zeta potential measurement;

 

d). Repeating the above procedures until approaching the final target pH automatically;

 

e) Saving and outputting complete data and the trend plot of zeta potential vs. pH;

 

f). Giving the isoelectric point if it is included in the setting pH range.

 

4) Download BAT-1 Autotitrator Flyer